- 靶点:
- SIRT3
- 简介:
- >>Nicotinate and nicotinamide metabolism;>>Metabolic pathways;>>Central carbon metabolism in cancer
- 基因名称:
- SIRT3
- 蛋白名称:
- NAD-dependent protein deacetylase sirtuin-3 mitochondrial
- Human Gene Id:
- 23410
- Human Swiss Prot No:
- Q9NTG7
- Mouse Swiss Prot No:
- Q8R104
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human SIRT3. AA range:350-399
- 特异性:
- SIRT3 Polyclonal Antibody detects endogenous levels of SIRT3 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度:
- 1 mg/ml
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- SIRT3;SIR2L3;NAD-dependent protein deacetylase sirtuin-3; mitochondrial;hSIRT3;Regulatory protein SIR2 homolog 3;SIR2-like protein 3
- 实测条带:
- 45kD
- 背景:
- This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq, Jul 2008],
- 功能:
- catalytic activity:NAD(+) + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.,cofactor:Binds 1 zinc ion per subunit.,function:NAD-dependent deacetylase. Despite some ability to deacetylate histones in vitro, it is unlikely in vivo.,PTM:Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.,similarity:Belongs to the sirtuin family.,similarity:Contains 1 deacetylase sirtuin-type domain.,tissue specificity:Widely expressed.,
- 细胞定位:
- Mitochondrion matrix .
- 组织表达:
- Widely expressed.
CD90 affects the biological behavior and energy metabolism level of gastric cancer cells by targeting the PI3K/AKT/HIF‑1α signaling pathway. Oncology Letters Oncol Lett. 2021 Mar;21(3):1-1 WB Human 1:1000 AGS cell
货号:YT4304
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- May 20-2022
- Cell-Based ELISA│解您多样本WB检测之困扰
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- 产品图片
- Western blot analysis of the lysates from HepG2 cells using SIRT3 antibody.