SERGEF Polyclonal Antibody
- 货号:YT4254
- 应用:WB;ELISA;IHC
- 种属:Human;Mouse
- 蛋白名称:
- Secretion-regulating guanine nucleotide exchange factor
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human SERGEF. AA range:179-228
- 特异性:
- SERGEF Polyclonal Antibody detects endogenous levels of SERGEF protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- SERGEF;DELGEF;GNEFR;Secretion-regulating guanine nucleotide exchange factor;Deafness locus-associated putative guanine nucleotide exchange factor;DelGEF;Guanine nucleotide exchange factor-related protein
- 背景:
- function:Probable guanine nucleotide exchange factor (GEF), which may be involved in the secretion process.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 7 RCC1 repeats.,subunit:Interacts with SEC5. The interaction occurs only in the presence of magnesium or manganese and is stimulated by dCTP or GTP.,
- 功能:
- function:Probable guanine nucleotide exchange factor (GEF), which may be involved in the secretion process.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 7 RCC1 repeats.,subunit:Interacts with SEC5. The interaction occurs only in the presence of magnesium or manganese and is stimulated by dCTP or GTP.,
- 细胞定位:
- Cytoplasm . Nucleus .
- Western blot analysis of SERGEF Antibody. The lane on the right is blocked with the SERGEF peptide.
- Western blot analysis of the lysates from HT-29 cells using SERGEF antibody.
- Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).