Sam 68 Polyclonal Antibody
- 货号：YT4209
- 应用：WB;ELISA
- 种属：Human;Mouse;Rat
- 蛋白名称：
- KH domain-containing RNA-binding signal transduction-associated protein 1
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human Sam 68. AA range:96-145
- 特异性：
- Sam 68 Polyclonal Antibody detects endogenous levels of Sam 68 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- KHDRBS1;SAM68;KH domain-containing; RNA-binding, signal transduction-associated protein 1;GAP-associated tyrosine phosphoprotein p62;Src-associated in mitosis 68 kDa protein;Sam68;p21 Ras GTPase-activating protein-associated p62;p68
- 背景：
- This gene encodes a member of the K homology domain-containing, RNA-binding, signal transduction-associated protein family. The encoded protein appears to have many functions and may be involved in a variety of cellular processes, including alternative splicing, cell cycle regulation, RNA 3'-end formation, tumorigenesis, and regulation of human immunodeficiency virus gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012],
- 功能：
- developmental stage:Isoform 3 is only expressed in growth-arrested cells.,domain:The KH domain is required for binding to RNA.,domain:The Pro-rich domains are flanked by Arg/Gly-rich motifs which can be asymmetric dimethylated on arginine residues to give the DMA/Gly-rich regions. Selective methlylation on these motifs can modulate protein-protein interactions.,function:Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase.,function:Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as
- 细胞定位：
- Nucleus . Cytoplasm . Membrane . Predominantly located in the nucleus but also located partially in the cytoplasm. .
- 组织表达：
- Ubiquitously expressed in all tissue examined. Isoform 1 is expressed at lower levels in brain, skeletal muscle, and liver whereas isoform 3 is intensified in skeletal muscle and in liver.
- Western Blot analysis of various cells using Sam 68 Polyclonal Antibody
- Western blot analysis of lysate from HUVEC cells, using Sam 68 antibody.
