• 靶点：
• RTCD1

• 基因名称：
• RTCA

• 蛋白名称：
• RNA 3'-terminal phosphate cyclase

• Human Gene Id：
• 8634

• Human Swiss Prot No：
• O00442

• Mouse Swiss Prot No：
• Q9D7H3

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human RTCD1. AA range:317-366

• 特异性：
• RTCD1 Polyclonal Antibody detects endogenous levels of RTCD1 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• RTCA;RPC;RPC1;RTC1;RTCD1;RNA 3'-terminal phosphate cyclase;RNA cyclase;RNA-3'-phosphate cyclase;RNA terminal phosphate cyclase domain-containing protein 1;RTC domain-containing protein 1

• 实测条带：
• 40kD

• 背景：
• RNA 3'-terminal phosphate cyclase(RTCA) Homo sapiens This gene encodes a member of the RNA 3'-phosphate cyclase family. The encoded protein plays a role in RNA metabolism by catalyzing the ATP-dependent conversion of the 3'-phosphate of RNA substrates to a 2',3'-cyclic phosphodiester. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012],

• 功能：
• catalytic activity:ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.,function:Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.,similarity:Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.,subunit:Monomer.,tissue specificity:Ubiquitous.,

• 细胞定位：
• Nucleus, nucleoplasm .

• 组织表达：
• Ubiquitous.

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs