Ribosomal Protein L27A Polyclonal Antibody

    • 货号:YT4103
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • Ribosomal Protein L27A
      • 简介:
      • >>Ribosome;>>Coronavirus disease - COVID-19
      • 基因名称:
      • RPL27A
      • 蛋白名称:
      • 60S ribosomal protein L27a
      • Human Gene Id:
      • 6157
      • Human Swiss Prot No:
      • P46776
      • Mouse Swiss Prot No:
      • P14115
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human RPL27A. AA range:99-148
      • 特异性:
      • Ribosomal Protein L27A Polyclonal Antibody detects endogenous levels of Ribosomal Protein L27A protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • RPL27A;60S ribosomal protein L27a
      • 实测条带:
      • 20kD
      • 背景:
      • Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, multiple processed pseudogenes derived from this gene are dispersed through the genome. [provided by RefSeq, Jul 2008],
      • 功能:
      • similarity:Belongs to the ribosomal protein L15P family.,
      • 细胞定位:
      • cytosol,ribosome,large ribosomal subunit,membrane,cytosolic large ribosomal subunit,
      • 组织表达:
      • Brain,Colon,Kidney,Lymph,
      • 产品图片
      • Western Blot analysis of various cells using Ribosomal Protein L27A Polyclonal Antibody diluted at 1:2000
      • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Immunofluorescence analysis of A549 cells, using RPL27A Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from COLO cells, using RPL27A Antibody. The lane on the right is blocked with the synthesized peptide.