• 靶点：
• RhoGEF p115

• 简介：
• >>Vascular smooth muscle contraction;>>Platelet activation;>>Regulation of actin cytoskeleton;>>Parathyroid hormone synthesis, secretion and action;>>Pathogenic Escherichia coli infection;>>Yersinia infection;>>Human cytomegalovirus infection;>>Pathways in cancer;>>Proteoglycans in cancer;>>Lipid and atherosclerosis

• 基因名称：
• ARHGEF1

• 蛋白名称：
• Rho guanine nucleotide exchange factor 1

• Human Gene Id：
• 9138

• Human Swiss Prot No：
• Q92888

• Mouse Gene Id：
• 16801

• Mouse Swiss Prot No：
• Q61210

• Rat Swiss Prot No：
• Q9Z1I6

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human ARHGEF1. AA range:162-211

• 特异性：
• RhoGEF p115 Polyclonal Antibody detects endogenous levels of RhoGEF p115 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• ARHGEF1;Rho guanine nucleotide exchange factor 1;115 kDa guanine nucleotide exchange factor;p115-RhoGEF;p115RhoGEF;Sub1.5

• 实测条带：
• 105kD

• 背景：
• Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008],

• 功能：
• domain:The DH domain is involved in interaction with CCPG1.,domain:The RGSL domain, also known as rgRGS domain, is necessary but not sufficient for GAP activity.,function:Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12.,PTM:Phosphorylated by PKCA.,sequence caution:Contaminating sequence. Sequence of unknown origin in the N-terminal part.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 RGSL (RGS-like) domain.,subcellular location:Translocated to the membrane by activ

• 细胞定位：
• Cytoplasm . Membrane . Translocated to the membrane by activated GNA13 or LPA stimulation.

• 组织表达：
• Ubiquitously expressed.

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