RGS12 Polyclonal Antibody
- 货号：YT4072
- 应用：WB;ELISA;IHC
- 种属：Human;Mouse;Rat
- 蛋白名称：
- Regulator of G-protein signaling 12
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human RGS12. AA range:141-190
- 特异性：
- RGS12 Polyclonal Antibody detects endogenous levels of RGS12 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- RGS12;Regulator of G-protein signaling 12;RGS12
- 背景：
- This gene encodes a member of the 'regulator of G protein signaling' (RGS) gene family. The encoded protein may function as a guanosine triphosphatase (GTPase)-activating protein as well as a transcriptional repressor. This protein may play a role in tumorigenesis. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their biological nature has not been determined. [provided by RefSeq, Jul 2008],
- 功能：
- function:Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 GoLoco domain.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 PID domain.,similarity:Contains 1 RGS domain.,similarity:Contains 2 RBD (Ras-binding) domains.,tissue specificity:Isoform 3 is brain specific.,
- 细胞定位：
- Nucleus . Cytoplasm . Cell projection, dendrite . Cell junction, synapse .; [Isoform 5]: Nucleus matrix . Also localized to discrete nuclear foci that are distinct from sites of RNA processing, PML nuclear bodies, and PcG domains. .
- 组织表达：
- Isoform 3 is brain specific.
- Western Blot analysis of 3T3 cells using RGS12 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western blot analysis of RGS12 Antibody. The lane on the right is blocked with the RGS12 peptide.
- Western blot analysis of the lysates from HUVECcells using RGS12 antibody.
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
