REPS1 Polyclonal Antibody
- 货号：YT4054
- 应用：WB;ELISA;IHC
- 种属：Human;Mouse
- 蛋白名称：
- RalBP1-associated Eps domain-containing protein 1
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human REPS1. AA range:597-646
- 特异性：
- REPS1 Polyclonal Antibody detects endogenous levels of REPS1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- REPS1;RalBP1-associated Eps domain-containing protein 1;RalBP1-interacting protein 1
- 背景：
- This gene encodes a signaling adaptor protein with two EH domains that interacts with proteins that participate in signaling, endocytosis and cytoskeletal changes. The encoded protein has been found in association with intersectin 1 and Src homology 3-domain growth factor receptor-bound 2-like (endophilin) interacting protein 1 when intersectin 1 was isolated from clathrin-coated pits. The encoded protein has also been shown to interact with amphiphysin, a cytoplasmic protein at the surface of synaptic vesicles. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014],
- 功能：
- function:May coordinate the cellular actions of activated EGF receptors and Ral-GTPases.,PTM:EGF stimulates phosphorylation on Tyr-residues.,similarity:Contains 1 EF-hand domain.,similarity:Contains 1 EH domain.,subunit:Homodimer (Potential). Interacts with RAB11FIP2. Interacts with RALBP1, CRK and GRB2. Binding to RALBP1 does not affect its Ral-binding activity. Forms a complex with the SH3 domains of CRK and GRB2 which may link it to an EGF-responsive tyrosine kinase.,
- 细胞定位：
- Membrane, clathrin-coated pit . Colocalize with ITSN1 at the plasma membrane in structures that are most probably clathrin-coated pits.
- 组织表达：
- Widely expressed with highest levels in heart and testis.
- Western blot analysis of REPS1 Antibody. The lane on the right is blocked with the REPS1 peptide.
- Western blot analysis of the lysates from K562 cells using REPS1 antibody.
- Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
