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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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RCL1 Polyclonal Antibody
- 货号:YT4033
- 应用:WB;IHC
- 种属:Human;Mouse;Rat
- 简介:
- >>Ribosome biogenesis in eukaryotes
- 蛋白名称:
- RNA 3'-terminal phosphate cyclase-like protein
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human RCL1. AA range:324-373
- 特异性:
- RCL1 Polyclonal Antibody detects endogenous levels of RCL1 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- RCL1;RNAC;RPC2;RPCL1;RTC2;HSPC338;RNA 3'-terminal phosphate cyclase-like protein
- 背景:
- function:Does not have cyclase activity. Plays a role in 40S-ribosomal-subunit biogenesis in the early pre-rRNA processing steps at sites A0, A1 and A2 that are required for proper maturation of the 18S RNA.,similarity:Belongs to the RNA 3'-terminal cyclase family. Type 2 subfamily.,
- 功能:
- function:Does not have cyclase activity. Plays a role in 40S-ribosomal-subunit biogenesis in the early pre-rRNA processing steps at sites A0, A1 and A2 that are required for proper maturation of the 18S RNA.,similarity:Belongs to the RNA 3'-terminal cyclase family. Type 2 subfamily.,
- 细胞定位:
- Nucleus, nucleolus .
- 组织表达:
- Eye,Seminom,Umbilical cord blood,
- Western Blot analysis of HUVEC cells using RCL1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western blot analysis of lysates from HUVEC cells, using RCL1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HepG2 cells using RCL1 antibody.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).