Rabaptin-5β Polyclonal Antibody
- 货号:YT3948
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Rab GTPase-binding effector protein 2
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human RABEP2. AA range:353-402
- 特异性:
- Rabaptin-5β Polyclonal Antibody detects endogenous levels of Rabaptin-5β protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- RABEP2;RABPT5B;Rab GTPase-binding effector protein 2;Rabaptin-5beta
- 背景:
- function:Plays a role in membrane trafficking and in homotypic early endosome fusion.,similarity:Belongs to the rabaptin family.,subunit:Heterodimer with RABGEF1. The dimer binds RAB5A that has been activated by GTP-binding.,
- 功能:
- function:Plays a role in membrane trafficking and in homotypic early endosome fusion.,similarity:Belongs to the rabaptin family.,subunit:Heterodimer with RABGEF1. The dimer binds RAB5A that has been activated by GTP-binding.,
- 细胞定位:
- Cytoplasm . Early endosome . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, cilium basal body .
- 组织表达:
- Hepatoma,Mammary gland,Testis,
- Western Blot analysis of various cells using Rabaptin-5β Polyclonal Antibody
- Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Western blot analysis of lysates from HeLa, Jurkat, 293, and HUVEC cells, using RABEP2 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HeLa cells using RABEP2 antibody.