Rab 35 Polyclonal Antibody
- 货号:YT3931
- 应用:WB;ELISA;IHC
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Ras-related protein Rab-35
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human RAB35. AA range:99-148
- 特异性:
- Rab 35 Polyclonal Antibody detects endogenous levels of Rab 35 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- RAB35;RAB1C;RAY;Ras-related protein Rab-35;GTP-binding protein RAY;Ras-related protein Rab-1C
- 背景:
- function:Possesses GTPase activity.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,
- 功能:
- function:Possesses GTPase activity.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,
- 细胞定位:
- Cell membrane ; Lipid-anchor ; Cytoplasmic side . Membrane, clathrin-coated pit . Cytoplasmic vesicle, clathrin-coated vesicle . Endosome . Melanosome . Present on sorting endosomes and recycling endosome tubules (PubMed:16950109). Tends to be enriched in PIP2-positive cell membrane domains (PubMed:16950109). During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis (PubMed:16950109). Identified in stage I to stage IV melanosomes (PubMed:17081065). .
- 组织表达:
- Brain,Muscle,Uterus,
![](/uploadfiles/PIC/yt3931-wb.jpg)
- Western Blot analysis of various cells using Rab 35 Polyclonal Antibody
![](/uploadfiles/PIC/yt3931_pic744018522.jpg)
- Western blot analysis of RAB35 Antibody. The lane on the right is blocked with the RAB35 peptide.
![](/uploadfiles/PIC/yt3931_pic1048618522.jpg)
- Western blot analysis of the lysates from HT-29 cells using RAB35 antibody.
![](/uploadfiles/PIC/4feiaicc.jpg)
- Immunohistochemical analysis of paraffin-embedded human lung cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).