PSMD12 Polyclonal Antibody
- 货号：YT3887
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse;Rat
- 简介：
- >>Proteasome;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Spinocerebellar ataxia;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Epstein-Barr virus infection
- 蛋白名称：
- 26S proteasome non-ATPase regulatory subunit 12
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human PSMD12. AA range:151-200
- 特异性：
- PSMD12 Polyclonal Antibody detects endogenous levels of PSMD12 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- PSMD12;26S proteasome non-ATPase regulatory subunit 12;26S proteasome regulatory subunit RPN5;26S proteasome regulatory subunit p55
- 背景：
- The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 3. Alternatively spliced transcript variants encoding
- 功能：
- function:Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.,similarity:Belongs to the proteasome subunit p55 family.,similarity:Contains 1 PCI domain.,subunit:Component of the PA700 complex.,
- 细胞定位：
- proteasome complex,nucleoplasm,cytoplasm,cytosol,proteasome regulatory particle,proteasome regulatory particle, lid subcomplex,membrane,proteasome accessory complex,nuclear proteasome complex,extracellular exosome,
- 组织表达：
- Brain,Muscle,Platelet,Testis,Tongue,
- Western Blot analysis of various cells using PSMD12 Polyclonal Antibody
.jpg)
- Western Blot analysis of HepG2 cells using PSMD12 Polyclonal Antibody
- Western blot analysis of lysates from HepG2 cells, using PSMD12 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HT-29 cells using PSMD12 antibody.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
