PIG-F Polyclonal Antibody
- 货号：YT3724
- 应用：IHC;IF;ELISA
- 种属：Human;Mouse
- 简介：
- >>Glycosylphosphatidylinositol (GPI)-anchor biosynthesis;>>Metabolic pathways
- 蛋白名称：
- Phosphatidylinositol-glycan biosynthesis class F protein
- 免疫原：
- Synthesized peptide derived from PIG-F . at AA range: 130-210
- 特异性：
- PIG-F Polyclonal Antibody detects endogenous levels of PIG-F protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- PIGF;Phosphatidylinositol-glycan biosynthesis class F protein;PIG-F;GPI11 homolog
- 背景：
- This gene encodes a protein involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor, a glycolipid containing three mannose molecules in its core backbone, is found on many blood cells where it serves to anchor proteins to the cell surface. The encoded protein and another GPI synthesis protein, PIGO, function in the transfer of ethanolaminephosphate to the third mannose in GPI. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008],
- 功能：
- function:Involved in GPI-anchor biosynthesis through the transfer of ethanolamine phosphate to the third mannose of GPI.,pathway:Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.,similarity:Belongs to the PIGF family.,subunit:Forms a complex with PIGG and PIGO. PIGF is required to stabilize PIGG and PIGO.,
- 细胞定位：
- Endoplasmic reticulum membrane ; Multi-pass membrane protein .
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
