PHKG1 Polyclonal Antibody
- 货号：YT3702
- 应用：WB;IHC
- 种属：Human;Rat;Mouse;
- 简介：
- >>Calcium signaling pathway;>>Insulin signaling pathway;>>Glucagon signaling pathway
- 蛋白名称：
- Phosphorylase b kinase gamma catalytic chain skeletal muscle isoform
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human PHKG1. AA range:241-290
- 特异性：
- PHKG1 Polyclonal Antibody detects endogenous levels of PHKG1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000;IHC 1:50-300
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- PHKG1;PHKG;Phosphorylase b kinase gamma catalytic chain; skeletal muscle isoform;Phosphorylase kinase subunit gamma-1
- 背景：
- This gene is a member of the Ser/Thr protein kinase family and encodes a protein with one protein kinase domain and two calmodulin-binding domains. This protein is the catalytic member of a 16 subunit protein kinase complex which contains equimolar ratios of 4 subunit types. The complex is a crucial glycogenolytic regulatory enzyme. This gene has two pseudogenes at chromosome 7q11.21 and one at chromosome 11p11.12. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012],
- 功能：
- catalytic activity:2 ATP + phosphorylase b = 2 ADP + phosphorylase a.,domain:The two calmodulin-binding domains appear to act in concert to bind a single molecule of calmodulin and are pseudosubstrate/autoinhibitory domains.,function:Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subunit:Polymer of 16 chains, four each of alpha, beta, gamma, and delta. Alpha and beta are regulatory chains, gamma is the catalytic chain, and delta is calmodulin.,
- 细胞定位：
- cytosol,phosphorylase kinase complex,
- 组织表达：
- PCR rescued clones,Salivary gland,Skeletal muscle,
- Western Blot analysis of various cells using PHKG1 Polyclonal Antibody
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- Western Blot analysis of HepG2 cells using PHKG1 Polyclonal Antibody
- Western blot analysis of the lysates from HUVECcells using PHKG1 antibody.
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
