PDLIM5 Polyclonal Antibody
- 货号：YT3647
- 应用：WB;IHC
- 种属：Human;Rat;Mouse;
- 蛋白名称：
- PDZ and LIM domain protein 5
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human PDLIM5. AA range:208-257
- 特异性：
- PDLIM5 Polyclonal Antibody detects endogenous levels of PDLIM5 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000;IHC 1:50-300
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- PDLIM5;ENH;L9;PDZ and LIM domain protein 5;Enigma homolog;Enigma-like PDZ and LIM domains protein
- 背景：
- This gene encodes a member of a family of proteins that possess a 100-amino acid PDZ domain at the N terminus and one to three LIM domains at the C-terminus. This family member functions as a scaffold protein that tethers protein kinases to the Z-disk in striated muscles. It is thought to function in cardiomyocyte expansion and in restraining postsynaptic growth of excitatory synapses. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012],
- 功能：
- caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,disease:May play a role in genetic susceptibility to schizophrenia. PDLIM5 is commonly increased in the brain of patients with bipolar disorder, schizophrenia, and major depression.,function:May play an important role in the heart development by scaffolding PKC to the Z-disk region.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 3 LIM zinc-binding domains.,subunit:Interacts with various PKC isoforms through the LIM domains. Interacts with actin and alpha-actinin through the PDZ domain.,tissue specificity:Heart and skeletal muscle specific.,
- 细胞定位：
- Cell junction, synapse, postsynaptic density . Cell junction, synapse, presynapse . Cell junction, synapse, postsynapse . Cytoplasm, cytosol . Detected both at presynaptic and postsynaptic sites, exclusively at excitatory synapses, but not inhibitory synapses, in hippocampal neurons. .
- 组织表达：
- Heart and skeletal muscle specific. Expression is commonly increased in the brain of patients with bipolar disorder, schizophrenia, and major depression.
- Western Blot analysis of A549 cells using PDLIM5 Polyclonal Antibody
- Western blot analysis of lysate from A549 cells, using PDLIM5 antibody.
- Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
