Pax-8 Polyclonal Antibody
- 货号：YT3602
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse;Rat
- 简介：
- >>Thyroid hormone synthesis;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Thyroid cancer
- 蛋白名称：
- Paired box protein Pax-8
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human Pax-8. AA range:145-194
- 特异性：
- Pax-8 Polyclonal Antibody detects endogenous levels of Pax-8 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- PAX8;Paired box protein Pax-8
- 背景：
- This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010],
- 功能：
- caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,developmental stage:In developing excretory system, during thyroid differentiation and in adult thyroid.,disease:Defects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue.,function:Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cell
- 组织表达：
- Expressed in the excretory system, thyroid gland and Wilms tumors.
- Western Blot analysis of various cells using Pax-8 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
- Immunohistochemistry analysis of Pax-8 antibody in paraffin-embedded human lung carcinoma tissue.
- Western blot analysis of lysate from HT-29 cells, using Pax-8 antibody.
