PARP-3 Polyclonal Antibody
- 货号：YT3595
- 应用：IHC;IF;ELISA
- 种属：Human;Rat;Mouse;
- 简介：
- >>Base excision repair;>>Apoptosis
- 蛋白名称：
- Poly [ADP-ribose] polymerase 3
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human PARP3. AA range:10-59
- 特异性：
- PARP-3 Polyclonal Antibody detects endogenous levels of PARP-3 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- PARP3;ADPRT3;ADPRTL3;Poly [ADP-ribose] polymerase 3;PARP-3;hPARP-3;ADP-ribosyltransferase diphtheria toxin-like 3;ARTD3;IRT1;NAD(+) ADP-ribosyltransferase 3;ADPRT-3;Poly[ADP-ribose] synthase 3;pADPRT-3
- 背景：
- The protein encoded by this gene belongs to the PARP family. These enzymes modify nuclear proteins by poly-ADP-ribosylation, which is required for DNA repair, regulation of apoptosis, and maintenance of genomic stability. This gene encodes the poly(ADP-ribosyl)transferase 3, which is preferentially localized to the daughter centriole throughout the cell cycle. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],
- 功能：
- catalytic activity:NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.,domain:According to PubMed:10329013 the N-terminal domain (54 amino acids) of isoform 2 is responsible for its centrosomal localization. The C-terminal region contains the catalytic domain.,function:Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. May link the DNA damage surveillance network to the mitotic fidelity checkpoint. Negatively influences the G1/S cell cycle progression without interfering with centrosome duplication. Binds DNA. May be involved in the regulation of PRC2 and PRC3 comple
- 细胞定位：
- Nucleus . Chromosome . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Almost exclusively localized in the nucleus and appears in numerous small foci and a small number of larger foci whereas a centrosomal location has not been detected (PubMed:16924674). In response to DNA damage, localizes to sites of double-strand break (PubMed:21270334). Preferentially localized to the daughter centriole (PubMed:10329013). .
- 组织表达：
- Widely expressed; the highest levels are in the kidney, skeletal muscle, liver, heart and spleen; also detected in pancreas, lung, placenta, brain, leukocytes, colon, small intestine, ovary, testis, prostate and thymus.
- Immunohistochemistry analysis of paraffin-embedded human brain, using PARP3 Antibody. The picture on the right is blocked with the synthesized peptide.
