PARP-2 Polyclonal Antibody
- 货号:YT3594
- 应用:WB;ELISA
- 种属:Human;Mouse
- 简介:
- >>Base excision repair;>>Apoptosis
- 蛋白名称:
- Poly [ADP-ribose] polymerase 2
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human PARP2. AA range:151-200
- 特异性:
- PARP-2 Polyclonal Antibody detects endogenous levels of PARP-2 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- PARP2;ADPRT2;ADPRTL2;Poly [ADP-ribose] polymerase 2;PARP-2;hPARP-2;ADP-ribosyltransferase diphtheria toxin-like 2;ARTD2;NAD(+) ADP-ribosyltransferase 2;ADPRT-2;Poly[ADP-ribose] synthase 2;pADPRT-2
- 背景:
- This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jul 2008],
- 功能:
- catalytic activity:NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.,function:Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.,PTM:Poly-ADP-ribosylated by PARP1.,similarity:Contains 1 PARP alpha-helical domain.,similarity:Contains 1 PARP catalytic domain.,subunit:Component of a base excision repair (BER) complex, containing at least XRCC1, PARP1, POLB and LIG3. Homo- and heterodimer with PARP1.,tissue specificity:Widely expressed, mainly in actively dividing tissues. The highest levels are in the brain, heart, pancreas, skeletal muscle and testis; also detected i
- 细胞定位:
- Nucleus . Chromosome . Recruited to DNA damage sites. .
- 组织表达:
- Widely expressed, mainly in actively dividing tissues (PubMed:10364231). The highest levels are in the brain, heart, pancreas, skeletal muscle and testis; also detected in kidney, liver, lung, placenta, ovary and spleen; levels are low in leukocytes, colon, small intestine, prostate and thymus (PubMed:10364231).
- Western Blot analysis of K562 cells using PARP-2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).