Oct-2 Polyclonal Antibody
- 货号:YT3231
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Monkey
- 简介:
- >>Herpes simplex virus 1 infection;>>Lipid and atherosclerosis
- 蛋白名称:
- POU domain class 2 transcription factor 2
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human OCT2. AA range:1-50
- 特异性:
- Oct-2 Polyclonal Antibody detects endogenous levels of 40818 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- POU2F2;OCT2;OTF2;POU domain; class 2, transcription factor 2;Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2;Octamer-binding protein 2;Oct-2;Octamer-binding transcription factor 2;OTF-2
- 背景:
- The protein encoded by this gene is a homeobox-containing transcription factor of the POU domain family. The encoded protein binds the octamer sequence 5'-ATTTGCAT-3', a common transcription factor binding site in immunoglobulin gene promoters. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011],
- 功能:
- function:Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.,similarity:Belongs to the POU transcription factor family. Class-2 subfamily.,similarity:Contains 1 homeobox DNA-binding domain.,similarity:Contains 1 POU-specific domain.,subunit:Interacts with NR3C1, AR and PGR.,tissue specificity:Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line Molt-4, but not in the T-cell line BW 5147.,
- 细胞定位:
- Cytoplasm . Nucleus.
- 组织表达:
- Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.
- Western Blot analysis of various cells using Oct-2 Polyclonal Antibody
- Immunofluorescence analysis of COS7 cells, using OCT2 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from COS7 cells, using OCT2 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).