NT5C1A Polyclonal Antibody
- 货号：YT3198
- 应用：WB;IF;ELISA
- 种属：Human;Mouse
- 简介：
- >>Purine metabolism;>>Pyrimidine metabolism;>>Nicotinate and nicotinamide metabolism;>>Metabolic pathways;>>Nucleotide metabolism
- 蛋白名称：
- Cytosolic 5'-nucleotidase 1A
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human NT5C1A. AA range:151-200
- 特异性：
- NT5C1A Polyclonal Antibody detects endogenous levels of NT5C1A protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- NT5C1A;Cytosolic 5'-nucleotidase 1A;cN1A;Cytosolic 5'-nucleotidase IA;cN-I;cN-IA
- 背景：
- Cytosolic nucleotidases, such as NT5C1A, dephosphorylate nucleoside monophosphates (Hunsucker et al., 2001 [PubMed 11133996]).[supplied by OMIM, Mar 2008],
- 功能：
- catalytic activity:A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate.,cofactor:Magnesium.,enzyme regulation:Activated by ADP.,function:Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides and has a broad substrate specificity. Helps to regulate adenosine levels in heart during ischemia and hypoxia.,similarity:Belongs to the 5'-nucleotidase type 3 family.,tissue specificity:Highly expressed in skeletal muscle. Detected at intermediate levels in heart, brain, kidney and pancreas.,
- 组织表达：
- Highly expressed in skeletal muscle. Detected at intermediate levels in heart, brain, kidney and pancreas.
- Immunofluorescence analysis of A549 cells, using NT5C1A Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from MCF-7 and HepG2 cells, using NT5C1A Antibody. The lane on the right is blocked with the synthesized peptide.
