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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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NSE2 Polyclonal Antibody
- 货号:YT3194
- 应用:WB;IHC;IF;ELISA
- 种属:Human
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human FAM84B. AA range:11-60
- 特异性:
- NSE2 Polyclonal Antibody detects endogenous levels of NSE2 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- FAM84B;BCMP101;NSE2;Protein FAM84B;Breast cancer membrane protein 101;Protein NSE2
- 背景:
- similarity:Belongs to the FAM84 family.,tissue specificity:Expressed in esophageal squamous cell carcinomas.,
- 功能:
- similarity:Belongs to the FAM84 family.,tissue specificity:Expressed in esophageal squamous cell carcinomas.,
- 细胞定位:
- cytoplasm,plasma membrane,
- 组织表达:
- Expressed in esophageal squamous cell carcinomas.
- Western Blot analysis of Jurkat cells using NSE2 Polyclonal Antibody
- Western blot analysis of lysates from Jurkat and HUVEC cells, using FAM84B Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).