• 靶点：
• NQO1

• 简介：
• >>Ubiquinone and other terpenoid-quinone biosynthesis;>>Metabolic pathways;>>Biosynthesis of cofactors;>>Pathways in cancer;>>Chemical carcinogenesis - reactive oxygen species;>>Hepatocellular carcinoma;>>Fluid shear stress and atherosclerosis

• 基因名称：
• NQO1

• 蛋白名称：
• NAD(P)H dehydrogenase [quinone] 1

• Human Gene Id：
• 1728

• Human Swiss Prot No：
• P15559

• Mouse Swiss Prot No：
• Q64669

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human NQO1. AA range:203-252

• 特异性：
• NQO1 Polyclonal Antibody detects endogenous levels of NQO1 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IF 1:50-200 ELISA: 1:10000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• NQO1;DIA4;NMOR1;NAD(P)H dehydrogenase [quinone] 1;Azoreductase;DT-diaphorase;DTD;Menadione reductase;NAD(P)H:quinone oxidoreductase 1;Phylloquinone reductase;Quinone reductase 1;QR1

• 实测条带：
• 31kD

• 背景：
• This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008],

• 功能：
• catalytic activity:NAD(P)H + a quinone = NAD(P)(+) + a hydroquinone.,cofactor:FAD.,enzyme regulation:Inhibited by dicoumarol.,function:The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.,induction:By dioxin.,mass spectrometry: PubMed:11735396,miscellaneous:Quinone reductase accepts electrons from both NADH and NADPH with equal efficiency.,polymorphism:The Ser-187 polymorphism may be linked to susceptibility to forms of cancers.,similarity:Belongs to the NAD(P)H dehydrogenase (quinone) family.,subunit:Homodimer.,

• 细胞定位：
• Cytoplasm, cytosol .

• 组织表达：
• Colon,Liver,Pooled,

Chemopreventive effects of atractylenolide II on mammary tumorigenesis via activating Nrf2-ARE pathway. Oncotarget Oncotarget. 2017 Sep 29; 8(44): 77500–77514 WB Rat mammary tissues
货号：YT3186

Continuous activation of Nrf2 and its target antioxidant enzymes leads to arsenite-induced malignant transformation of human bronchial epithelial cells. TOXICOLOGY AND APPLIED PHARMACOLOGY Toxicol Appl Pharm. 2015 Dec;289:231 WB Human HBE cell
货号：YT3186

Curcumin protects against methylmercury-induced cytotoxicity in primary rat astrocytes by activating the Nrf2/ARE pathway independently of PKCδ. TOXICOLOGY Toxicology. 2019 Sep;425:152248 WB Rat astrocytes
货号：YT3186

Zhang, Hong, et al. "Therapeutic potential of bixin in PM2. 5 particles-induced lung injury in an Nrf2-dependent manner." Free Radical Biology and Medicine 126 (2018): 166-176.
货号：YT3186

Berberine hydrochloride alleviates imatinib mesylate – induced cardiotoxicity through the inhibition of Nrf2-dependent ferroptosis Food & Function Jie Zhang IF,WB Rat H9c2 cells
货号：YT3186

lncRNA HOTAIR promotes ROS generation and NLRP3 inflammasome activation by inhibiting Nrf2 in diabetic retinopathy. MEDICINE Wenjun Gou WB Human 1:1000 Human retinal endothelial cells (HRECs)
货号：YT3186

Sunitinib induces cardiotoxicity through modulating oxidative stress and Nrf2-dependent ferroptosis in vitro and in vivo CHEMICO-BIOLOGICAL INTERACTIONS Dongjie Li WB Mouse,Rat heart tissue H9c2 cell
货号：YT3186

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs