Nkx-3.1 Polyclonal Antibody
- 货号：YT3142
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse
- 简介：
- >>Pathways in cancer;>>Prostate cancer
- 蛋白名称：
- Homeobox protein Nkx-3.1
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human NKX3-1. AA range:1-50
- 特异性：
- Nkx-3.1 Polyclonal Antibody detects endogenous levels of Nkx-3.1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- NKX3-1;NKX3.1;NKX3A;Homeobox protein Nkx-3.1;Homeobox protein NK-3 homolog A
- 背景：
- This gene encodes a homeobox-containing transcription factor. This transcription factor functions as a negative regulator of epithelial cell growth in prostate tissue. Aberrant expression of this gene is associated with prostate tumor progression. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jan 2012],
- 功能：
- alternative products:Additional isoforms seem to exist,disease:NKX3-1 has been thought to be one of the target gene of the 8p21 loss of heterozygosity, common in prostate cancer, but neither disruption of the coding region of the gene, nor mutations have been found in prostate cancer.,function:Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Could play an important role in regulating proliferation of glandular epithelium and in the formation of ducts in prostate.,induction:By androgens and, in the LNCAP cell line, by estrogens. Androgenic control may be lost in prostate cancer cells during tumor progression from an androgen-dependent to an androgen-independent phase.,similarity:Belongs to the NK-3 homeobox family.,similarity:Contains 1 homeobox DNA-binding domain.,subunit:Interacts with serum response f
- 组织表达：
- Highly expressed in the prostate and, at a lower level, in the testis.
- Western Blot analysis of various cells using Nkx-3.1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NKX3.1 Antibody. The picture on the right is blocked with the synthesized peptide.
