Nicalin Polyclonal Antibody

    • 货号:YT3124
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • Nicalin
      • 基因名称:
      • NCLN
      • 蛋白名称:
      • Nicalin
      • Human Swiss Prot No:
      • Q969V3
      • Mouse Swiss Prot No:
      • Q8VCM8
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human NCLN. AA range:474-523
      • 特异性:
      • Nicalin Polyclonal Antibody detects endogenous levels of Nicalin protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • NCLN;Nicalin;Nicastrin-like protein
      • 实测条带:
      • 63kD
      • 背景:
      • function:May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning.,similarity:Belongs to the nicastrin family.,subunit:Interacts with NOMO2. Due to the strong similarity between NOMO1, NOMO2 and NOMO3, it probably also interacts with NOMO1 and NOMO3. Participates in a large protein complex, which is not related to the gamma-secretase complex.,tissue specificity:Highly expressed in pancreas and skeletal muscle and, at lower levels, in heart.,
      • 功能:
      • function:May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning.,similarity:Belongs to the nicastrin family.,subunit:Interacts with NOMO2. Due to the strong similarity between NOMO1, NOMO2 and NOMO3, it probably also interacts with NOMO1 and NOMO3. Participates in a large protein complex, which is not related to the gamma-secretase complex.,tissue specificity:Highly expressed in pancreas and skeletal muscle and, at lower levels, in heart.,
      • 细胞定位:
      • Endoplasmic reticulum membrane ; Single-pass membrane protein .
      • 组织表达:
      • Highly expressed in pancreas and skeletal muscle and, at lower levels, in heart.
      • 产品图片
      • Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Western blot analysis of lysates from HepG2 and HUVEC cells, using NCLN Antibody. The lane on the right is blocked with the synthesized peptide.