Neuropsin Polyclonal Antibody
- 货号：YT3076
- 应用：WB;ELISA;IHC
- 种属：Human;Mouse
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human OPN5. AA range:251-300
- 特异性：
- Neuropsin Polyclonal Antibody detects endogenous levels of Neuropsin protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- OPN5;GPR136;PGR12;TMEM13;Opsin-5;G-protein coupled receptor 136;G-protein coupled receptor PGR12;Neuropsin;Transmembrane protein 13
- 背景：
- Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. This opsin gene is expressed in the eye, brain, testes, and spinal cord. This gene belongs to the seven-exon subfamily of mammalian opsin genes that includes peropsin (RRH) and retinal G protein coupled receptor (RGR). Like these other seven-exon opsin genes, this family member may encode a protein with photoisomerase activity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010],
- 功能：
- caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,PTM:It is uncertain whether Cys-315 or Cys-316 is palmitoylated.,similarity:Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.,tissue specificity:Detected in brain and retina and cell lines derived from neural retina.,
- 细胞定位：
- Cell membrane ; Multi-pass membrane protein .
- 组织表达：
- Detected in brain and retina and cell lines derived from neural retina.
- Western Blot analysis of Jurkat cells using Neuropsin Polyclonal Antibody
- Immunofluorescence analysis of HeLa cells, using OPN5 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from Jurkat cells, using OPN5 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HUVECcells using OPN5 antibody.
- Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
