Neuroglycan C Polyclonal Antibody

    • 货号:YT3066
    • 应用:WB;IHC
    • 种属:Human;Mouse;Rat
      • 靶点:
      • Neuroglycan C
      • 基因名称:
      • CSPG5
      • 蛋白名称:
      • Chondroitin sulfate proteoglycan 5
      • Human Swiss Prot No:
      • O95196
      • Mouse Swiss Prot No:
      • Q71M36
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human CSPG5. AA range:211-260
      • 特异性:
      • Neuroglycan C Polyclonal Antibody detects endogenous levels of Neuroglycan C protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500-2000;IHC 1:50-300
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • CSPG5;CALEB;NGC;Chondroitin sulfate proteoglycan 5;Acidic leucine-rich EGF-like domain-containing brain protein;Neuroglycan C
      • 实测条带:
      • 60kD
      • 背景:
      • The protein encoded by this gene is a proteoglycan that may function as a neural growth and differentiation factor. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011],
      • 功能:
      • developmental stage:Expressed in brain of 3 months, 5 and 10-year-old individuals.,function:May function as a growth and differentiation factor involved in neuritogenesis. May induce ERBB3 activation.,miscellaneous:Different forms of various molecular weight have been observed. Such forms are possibly due to different levels of glycosylation, phosphorylation and/or protein cleavage.,PTM:N-glycosylated.,PTM:O-glycosylated; contains chondroitin sulfate glycans. Part-time proteoglycan, expressed in part as a proteoglycan exhibiting chondroitin sulfate glycans and in part as a non-proteoglycan form. The relative amount of both forms depends on tissues and tissues maturation.,PTM:Phosphorylated; in intracellular and extracellular parts.,similarity:Contains 1 EGF-like domain.,subcellular location:In neurons, localizes to synaptic junctions (By similarity). Also detected in the endoplasmic reti
      • 细胞定位:
      • Cell membrane ; Single-pass type I membrane protein . Cell junction, synapse, synaptic cell membrane ; Single-pass type I membrane protein . Endoplasmic reticulum membrane ; Single-pass type I membrane protein . Golgi apparatus membrane ; Single-pass type I membrane protein . Cell surface . In neurons, localizes to synaptic junctions. Also detected in the endoplasmic reticulum and the Golgi. Partially enriched in lipid rafts. .
      • 组织表达:
      • Restricted to brain (at protein level).
      • 产品图片
      • Western Blot analysis of various cells using Neuroglycan C Polyclonal Antibody
      • Western Blot analysis of HeLa cells using Neuroglycan C Polyclonal Antibody
      • Western blot analysis of lysates from COLO and HeLa cells, using CSPG5 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).