NDUFA8 Polyclonal Antibody
- 货号：YT3010
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse
- 简介：
- >>Oxidative phosphorylation;>>Metabolic pathways;>>Thermogenesis;>>Retrograde endocannabinoid signaling;>>Non-alcoholic fatty liver disease;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Chemical carcinogenesis - reactive oxygen species;>>Diabetic cardiomyopathy
- 蛋白名称：
- NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human NDUFA8. AA range:109-158
- 特异性：
- NDUFA8 Polyclonal Antibody detects endogenous levels of NDUFA8 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- NDUFA8;NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8;Complex I-19kD;CI-19kD;Complex I-PGIV;CI-PGIV;NADH-ubiquinone oxidoreductase 19 kDa subunit
- 背景：
- The protein encoded by this gene belongs to the complex I 19 kDa subunit family. Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays an important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015],
- 功能：
- function:Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.,similarity:Belongs to the complex I NDUFA8 subunit family.,similarity:Contains 2 CHCH domains.,subunit:Complex I is composed of 45 different subunits.,
- 细胞定位：
- Mitochondrion inner membrane ; Peripheral membrane protein . Mitochondrion intermembrane space . Mitochondrion .
- 组织表达：
- Kidney,Lymph,Thalamus,
- Western Blot analysis of various cells using NDUFA8 Polyclonal Antibody diluted at 1:1000
- Immunohistochemistry analysis of paraffin-embedded human pancreas tissue, using NDUFA8 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HUVEC, MCF-7, Jurkat, HepG2, and 293 cells, using NDUFA8 Antibody. The lane on the right is blocked with the synthesized peptide.
