Myt 1 Polyclonal Antibody
- 货号：YT2958
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse
- 简介：
- >>Cell cycle;>>Oocyte meiosis;>>Progesterone-mediated oocyte maturation
- 蛋白名称：
- Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human PKMYT1. AA range:49-98
- 特异性：
- Myt 1 Polyclonal Antibody detects endogenous levels of Myt 1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- PKMYT1;MYT1;Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase;Myt1 kinase
- 背景：
- This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012],
- 功能：
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The membrane-association motif is essential for the localization to membrane of Golgi stack. According to some authors, it is a transmembrane domain; the existence of a transmembrane region is however unproven.,enzyme regulation:Negatively regulated by hyperphosphorylation during mitosis. The hyperphosphorylated form does not associate with CCNB1-CDC2 complexes. The PLK1 protein kinase may be required for mitotic phosphorylation.,function:Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the cdc2 kinase specifically when cdc2 is complexed to cyclins. Mediates phosphorylation of cdc2 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of cdc2 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be ind
- 细胞定位：
- Endoplasmic reticulum membrane ; Peripheral membrane protein . Golgi apparatus membrane ; Peripheral membrane protein .
- 组织表达：
- Brain,Epithelium,PCR rescued clones,
- Western Blot analysis of various cells using Myt 1 Polyclonal Antibody diluted at 1:2000
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MYT1 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HeLa cells, using MYT1 Antibody. The lane on the right is blocked with the synthesized peptide.
