Myomesin-2 Polyclonal Antibody
- 货号：YT2947
- 应用：WB;ELISA;IHC
- 种属：Human;Mouse;Monkey
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human MYOM2. AA range:612-661
- 特异性：
- Myomesin-2 Polyclonal Antibody detects endogenous levels of Myomesin-2 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- MYOM2;Myomesin-2;165 kDa connectin-associated protein;165 kDa titin-associated protein;M-protein;Myomesin family member 2
- 背景：
- The giant protein titin, together with its associated proteins, interconnects the major structure of sarcomeres, the M bands and Z discs. The C-terminal end of the titin string extends into the M line, where it binds tightly to M-band constituents of apparent molecular masses of 190 kD and 165 kD. The predicted MYOM2 protein contains 1,465 amino acids. Like MYOM1, MYOM2 has a unique N-terminal domain followed by 12 repeat domains with strong homology to either fibronectin type III or immunoglobulin C2 domains. Protein sequence comparisons suggested that the MYOM2 protein and bovine M protein are identical. [provided by RefSeq, Jul 2008],
- 功能：
- function:Major component of the vertebrate myofibrillar M band. Binds myosin, titin, and light meromyosin. This binding is dose dependent.,similarity:Contains 5 fibronectin type-III domains.,similarity:Contains 5 Ig-like C2-type (immunoglobulin-like) domains.,subunit:Interacts with TTN/titin.,
- 细胞定位：
- Cytoplasm, myofibril, sarcomere, M line .
- 组织表达：
- PNS,Skeletal muscle,
- Western Blot analysis of various cells using Myomesin-2 Polyclonal Antibody diluted at 1:500
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- Western Blot analysis of HT29 cells using Myomesin-2 Polyclonal Antibody diluted at 1:500
- Western blot analysis of lysates from COS7 and HT-29 cells, using MYOM2 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
