MYH14 Polyclonal Antibody
- 货号：YT2933
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse
- 简介：
- >>Vascular smooth muscle contraction;>>Tight junction;>>Regulation of actin cytoskeleton;>>Pathogenic Escherichia coli infection
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human MYH14. AA range:1051-1100
- 特异性：
- MYH14 Polyclonal Antibody detects endogenous levels of MYH14 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- MYH14;KIAA2034;FP17425;Myosin-14;Myosin heavy chain 14;Myosin heavy chain; non-muscle IIc;Non-muscle myosin heavy chain IIc;NMHC II-C
- 背景：
- This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-14 (MYO14). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene result in one form of autosomal dominant hearing impairment. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011],
- 功能：
- disease:Defects in MYH14 are the cause of non-syndromic sensorineural deafness autosomal dominant type 4 (DFNA4) [MIM:600652]. DFNA4 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.,domain:The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.,function:Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping.,sequence caution:Translation N-terminally extended.,similarity:Contains 1 IQ domain.,similarity:Contains 1 myosin head-like domain.,subunit:Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light cha
- 细胞定位：
- stress fiber,cytosol,brush border,membrane,myosin complex,myosin II complex,axon,growth cone,actomyosin,myelin sheath,extracellular exosome,myosin II filament,
- 组织表达：
- High levels of expression are found in brain (highest in corpus callosum), heart, kidney, liver, lung, small intestine, colon and skeletal muscle. Expression is low in organs composed mainly of smooth muscle, such as aorta, uterus and urinary bladder. No detectable expression is found in thymus, spleen, placenta and lymphocytes.
- Western Blot analysis of various cells using MYH14 Polyclonal Antibody diluted at 1:1000
- Western blot analysis of lysates from 293 cells, using MYH14 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HeLa cells using MYH14 antibody.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
