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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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MLTK Polyclonal Antibody
- 货号:YT2790
- 应用:WB;IHC
- 种属:Human;Mouse
- 简介:
- >>MAPK signaling pathway
- 蛋白名称:
- Mitogen-activated protein kinase kinase kinase MLT
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human MLTK. AA range:701-750
- 特异性:
- MLTK Polyclonal Antibody detects endogenous levels of MLTK protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- MLTK;ZAK;HCCS4;Mitogen-activated protein kinase kinase kinase MLT;Human cervical cancer suppressor gene 4 protein;HCCS-4;Leucine zipper- and sterile alpha motif-containing kinase;MLK-like mitogen-activated protein triple kinase;Mixe
- 背景:
- This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008],
- 功能:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by phosphorylation by PKN1 and autophosphorylation on Thr-161 and Ser-165.,function:Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2. Isoform 1, but not isoform 2, causes cell shrinkage and disruption of actin stress fibers. Isoform 1 may have role in neoplastic cell transformation and cancer development. Isoform 1, but not isoform 2, phosphorylates histone H3 at 'Ser-28'.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,subcellular location:Tr
- 细胞定位:
- Cytoplasm . Nucleus . Translocates to the nucleus upon ultraviolet B irradiation. .
- 组织表达:
- Ubiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for liver, in which isoform 1 is more highly expressed.
![](/uploadfiles/PIC/yt2790-wb.jpg)
- Western Blot analysis of various cells using MLTK Polyclonal Antibody diluted at 1:1000
![](/uploadfiles/PIC/yt2790_pic582218522.jpg)
- Immunofluorescence analysis of HepG2 cells, using MLTK Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt2790_pic582318522.jpg)
- Western blot analysis of lysates from HeLa cells, using MLTK Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/252feilinaicc.jpg)
- Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).