MLK4 Polyclonal Antibody

    • 货号:YT2787
    • 应用:WB;ELISA;IHC
    • 种属:Human;Mouse
      • 靶点:
      • MLK4
      • 基因名称:
      • MLK4
      • 蛋白名称:
      • Mitogen-activated protein kinase kinase kinase MLK4
      • Human Swiss Prot No:
      • Q5TCX8
      • Mouse Swiss Prot No:
      • Q8VDG6
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human MAP3KL4. AA range:631-680
      • 特异性:
      • MLK4 Polyclonal Antibody detects endogenous levels of MLK4 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • MLK4;KIAA1804;Mitogen-activated protein kinase kinase kinase MLK4;Mixed lineage kinase 4
      • 实测条带:
      • 110kD
      • 背景:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation.,function:Activates the JUN N-terminal pathway.,PTM:Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH3 domain.,subunit:Homodimer.,
      • 功能:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation.,function:Activates the JUN N-terminal pathway.,PTM:Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH3 domain.,subunit:Homodimer.,
      • 细胞定位:
      • intracellular,
      • 组织表达:
      • Brain,Heart,
      • 产品图片
      • Western Blot analysis of various cells using MLK4 Polyclonal Antibody
      • Western blot analysis of lysates from HT-29 cells, using MAP3KL4 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from HepG2 cells using MAP3KL4 antibody.
      • Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).