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- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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MLK3 Polyclonal Antibody
- 货号:YT2786
- 应用:IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 简介:
- >>MAPK signaling pathway;>>Non-alcoholic fatty liver disease
- 蛋白名称:
- Mitogen-activated protein kinase kinase kinase 11
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human MLK3. AA range:640-689
- 特异性:
- MLK3 Polyclonal Antibody detects endogenous levels of MLK3 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- MAP3K11;MLK3;PTK1;SPRK;Mitogen-activated protein kinase kinase kinase 11;Mixed lineage kinase 3;Src-homology 3 domain-containing proline-rich kinase
- 背景:
- The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq, Jul 2008],
- 功能:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation.,function:Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1). Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.,PTM:Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP
- 细胞定位:
- Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Location is cell cycle dependent.
- 组织表达:
- Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.
![](/uploadfiles/PIC/yt2786_pic422818522.jpg)
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MLK3 Antibody. The picture on the right is blocked with the synthesized peptide.