- 靶点：
- MITF
- 简介：
- >>Mitophagy - animal;>>Osteoclast differentiation;>>Melanogenesis;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Melanoma
- 基因名称：
- MITF
- 蛋白名称：
- Microphthalmia-associated transcription factor
- Human Gene Id：
- 4286
- Human Swiss Prot No：
- O75030
- Mouse Gene Id：
- 17342
- Mouse Swiss Prot No：
- Q08874
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human MITF. AA range:151-200
- 特异性：
- MITF Polyclonal Antibody detects endogenous levels of MITF protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度：
- 1 mg/ml
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- MITF;BHLHE32;Microphthalmia-associated transcription factor;Class E basic helix-loop-helix protein 32;bHLHe32
- 实测条带：
- 52kD
- 背景：
- This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],
- 功能：
- alternative products:The X2-type isoforms differ from the X1-type isoforms by the absence of a 6 residue insert,disease:Defects in MITF are a cause of Waardenburg syndrome type 2 with ocular albinism (WS2-OA) [MIM:103470]. It is an ocular albinism with sensorineural deafness.,disease:Defects in MITF are the cause of Tietz syndrome [MIM:103500]. It is an autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete.,disease:Defects in MITF are the cause of Waardenburg syndrome type 2A (WS2A) [MIM:193510]. It is a dominant inherited disorder characterized by sensorineural hearing loss and patches of depigmentation. The features show variable expression and penetrance.,function:Transcription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') foun
- 细胞定位：
- Nucleus . Cytoplasm . Found exclusively in the nucleus upon phosphorylation. .
- 组织表达：
- Expressed in melanocytes (at protein level). ; [Isoform A2]: Expressed in the retinal pigment epithelium, brain, and placenta (PubMed:9647758). Expressed in the kidney (PubMed:9647758, PubMed:10578055). ; [Isoform C2]: Expressed in the kidney and retinal pigment epithelium. ; [Isoform H1]: Expressed in the kidney. ; [Isoform H2]: Expressed in the kidney. ; [Isoform M1]: Expressed in melanocytes. ; [Isoform Mdel]: Expressed in melanocytes.
Hydroxyurea regulates the development and survival of B16 Melanoma Cells by upregulating MiR-7013-3p. International Journal of Medical Sciences Int J Med Sci. 2021; 18(8): 1877–1885 WB Mouse B16 melanoma cells
货号：YT2769
Flower extracts from Paeonia decomposita and Paeonia ostii inhibit melanin synthesis via cAMP‐CREB‐associated melanogenesis signaling pathways in murine B16 melanoma cells. JOURNAL OF FOOD BIOCHEMISTRY 2019 Jan 24 WB Mouse 1:3000–5000 B16 melanoma cell
货号：YT2769
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- Antibody-FAQs
- 产品图片
- Western Blot analysis of hela cells using primary antibody diluted at 1:500. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western Blot analysis of various cells using MITF Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
- Immunofluorescence analysis of HeLa cells, using MITF Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemistry analysis of paraffin-embedded human skin tissue, using MITF Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HepG2 cells, using MITF Antibody. The lane on the right is blocked with the synthesized peptide.
