• 靶点：
• MIF

• 简介：
• >>Tyrosine metabolism;>>Phenylalanine metabolism;>>Metabolic pathways

• 基因名称：
• MIF

• 蛋白名称：
• Macrophage migration inhibitory factor

• Human Gene Id：
• 4282

• Human Swiss Prot No：
• P14174

• Mouse Gene Id：
• 17319

• Mouse Swiss Prot No：
• P34884

• Rat Gene Id：
• 81683

• Rat Swiss Prot No：
• P30904

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human MIF. AA range:25-74

• 特异性：
• MIF Polyclonal Antibody detects endogenous levels of MIF protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• IF 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• MIF;GLIF;MMIF;Macrophage migration inhibitory factor;MIF;Glycosylation-inhibiting factor;GIF;L-dopachrome isomerase;L-dopachrome tautomerase;Phenylpyruvate tautomerase

• 实测条带：
• 12kD

• 背景：
• This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008],

• 功能：
• catalytic activity:Keto-phenylpyruvate = enol-phenylpyruvate.,disease:Genetic variations in MIF are associated with susceptibility to systemic juvenile rheumatoid arthritis [MIM:604302]. Systemic juvenile rheumatoid arthritis is juvenile chronic arthritis associated with severe, debilitating, extraarticular features, and occasionally fatal complications. Despite medical treatment, many children still experience early joint destruction, necessitating surgical replacement.,function:The expression of MIF at sites of inflammation suggest a role for the mediator in regulating the function of macrophage in host defense. Also acts as a phenylpyruvate tautomerase.,similarity:Belongs to the MIF family.,subunit:Homotrimer. Interacts with COPS5 and BNIPL.,

• 细胞定位：
• Secreted . Cytoplasm . Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. .

• 组织表达：
• Brain,Lens,Liver,Lung,Platelet,Skin

LC3-II may mediate ATR-induced mitophagy in dopaminergic neurons through SQSTM1/p62 pathway. ACTA BIOCHIMICA ET BIOPHYSICA SINICA 2018 Oct 01 WB Rat 1:1000 substantia nigra (SNpc) PC-12 cell
货号：YT2761

MIF, secreted by human hepatic sinusoidal endothelial cells, promotes chemotaxis and outgrowth of colorectal cancer in liver prometastasis. Oncotarget Oncotarget. 2015 Sep 8; 6(26): 22410–22423 IHC Human 1:100 CRC tissue
货号：YT2761

Sulforaphane effectively inhibits HBV by altering Treg/Th17 immune balance and the MIF-macrophages polarizing axis in vitro and in vivo VIRUS RESEARCH Ruqing Xu IHC Mouse 1:200 liver tissue
货号：YT2761

Tozasertib activates anti-tumor immunity through decreasing regulatory T cells in melanoma NEOPLASIA Qiaoling Wang IF Mouse B16F10 cell-Xenograft
货号：YT2761

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