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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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MGMT Polyclonal Antibody
- 货号:YT2750
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Rat
- 蛋白名称:
- Methylated-DNA--protein-cysteine methyltransferase
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human MGMT. AA range:1-50
- 特异性:
- MGMT Polyclonal Antibody detects endogenous levels of MGMT protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- MGMT;Methylated-DNA--protein-cysteine methyltransferase;6-O-methylguanine-DNA methyltransferase;MGMT;O-6-methylguanine-DNA-alkyltransferase
- 背景:
- Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015],
- 功能:
- catalytic activity:DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.,cofactor:Binds 1 zinc ion.,function:Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.,similarity:Belongs to the MGMT family.,
- 组织表达:
- Cerebellum,Epithelium,Placenta,
![](/uploadfiles/PIC/yt2750-wb.jpg)
- Western Blot analysis of various cells using MGMT Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
![](/uploadfiles/PIC/yt2750-wb(2).jpg)
- Western Blot analysis of Jurkat cells using MGMT Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
![](/uploadfiles/PIC/yt2750_pic631818522.jpg)
- Western blot analysis of lysates from Jurkat cells, using MGMT Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/19-ihcspleenn20220713.jpg)
- Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).