MARCH5 Polyclonal Antibody
- 货号：YT2646
- 应用：IF;WB;ELISA
- 种属：Human;Mouse;Rat
- 蛋白名称：
- E3 ubiquitin-protein ligase MARCH5
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human MARCH5. AA range:21-70
- 特异性：
- MARCH5 Polyclonal Antibody detects endogenous levels of 40607 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000 IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- MARCH5;RNF153;E3 ubiquitin-protein ligase MARCH5;Membrane-associated RING finger protein 5;Membrane-associated RING-CH protein V;MARCH-V;Mitochondrial ubiquitin ligase;MITOL;RING finger protein 153
- 背景：
- MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).[supplied by OMIM, Mar 2008],
- 功能：
- domain:The RING-CH-type zinc finger domain is required for E3 ligase activity.,function:Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology. Promotes ubiquitination of DRP1. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 RING-CH-type zinc finger.,subunit:Interacts with MTFN2 and ubiquitinated forms of DRP1.,
- 细胞定位：
- Mitochondrion outer membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Authors show that the protein can be detected in endoplasmic reticulum (PubMed:14722266). Authors (PubMed:16874301) show its presence only in mitochondria (PubMed:16874301). .
- 组织表达：
- Expressed in brain, heart, liver, lung, spleen, stomach, testis, skeletal and muscle.
- Immunofluorescence analysis of Hela cell. 1,MARCH5 Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
- Immunofluorescence analysis of A549 cells, using MARCH5 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night
