MAN1B1 Polyclonal Antibody
- 货号：YT2633
- 应用：WB;ELISA
- 种属：Human;Rat;Mouse;
- 简介：
- >>N-Glycan biosynthesis;>>Various types of N-glycan biosynthesis;>>Metabolic pathways;>>Protein processing in endoplasmic reticulum
- 蛋白名称：
- Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
- 免疫原：
- Synthesized peptide derived from MAN1B1 . at AA range: 100-180
- 特异性：
- MAN1B1 Polyclonal Antibody detects endogenous levels of MAN1B1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- MAN1B1;Endoplasmic reticulum mannosyl-oligosaccharide 1;2-alpha-mannosidase;ER alpha-1,2-mannosidase;ER mannosidase 1;ERMan1;Man9GlcNAc2-specific-processing alpha-mannosidase;Mannosidase alpha class 1B member 1
- 背景：
- This gene encodes an enzyme belonging to the glycosyl hydrolase 47 family. This enzyme functions in N-glycan biosynthesis, and is a class I alpha-1,2-mannosidase that specifically converts Man9GlcNAc to Man8GlcNAc isomer B. It is required for N-glycan trimming to Man5-6GlcNAc2 in the endoplasmic-reticulum-associated degradation pathway. Mutations in this gene cause autosomal-recessive intellectual disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 11. [provided by RefSeq, Dec 2011],
- 功能：
- catalytic activity:Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man(9)(GlcNAc)(2).,cofactor:Calcium.,enzyme regulation:Inhibited by both 1-deoxymannojirimycin and kifunensine.,function:Involved in the maturation of Asn-linked oligosaccharides. Trim a single alpha-1,2-linked mannose residue from Man(9)GlcNAc(2) to produce Man(8)GlcNAc(2). The only product is the Man(8)GlcNAc(2) isomer B, the form lacking the middle-arm terminal alpha 1,2-mannose. It may be involved in glycoprotein quality control since it is important to target misfolded glycoproteins for degradation.,pathway:Protein modification; protein glycosylation.,similarity:Belongs to the glycosyl hydrolase 47 family.,tissue specificity:Widely expressed.,
- 细胞定位：
- Endoplasmic reticulum membrane ; Single-pass type II membrane protein .
- Western Blot analysis of 3T3 cells using MAN1B1 Polyclonal Antibody
- Western blot analysis of MAN1B1 Antibody. The lane on the right is blocked with the MAN1B1 peptide.
