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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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LZK Polyclonal Antibody
- 货号:YT2610
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 简介:
- >>MAPK signaling pathway
- 蛋白名称:
- Mitogen-activated protein kinase kinase kinase 13
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human M3K13. AA range:151-200
- 特异性:
- LZK Polyclonal Antibody detects endogenous levels of LZK protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- MAP3K13;LZK;Mitogen-activated protein kinase kinase kinase 13;Leucine zipper-bearing kinase;Mixed lineage kinase;MLK
- 背景:
- The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase contains a dual leucine-zipper motif, and has been shown to form dimers/oligomers through its leucine-zipper motif. This kinase can phosphorylate and activate MAPK8/JNK, MAP2K7/MKK7, which suggests a role in the JNK signaling pathway. [provided by RefSeq, Jul 2008],
- 功能:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by autophosphorylation and homodimerization.,function:Activates the JUN N-terminal pathway through activation of the MAP kinase kinase MAP2K7. Acts synergistically with PRDX3 to regulate the activation of NF-kappa-B in the cytosol. This activation is kinase-dependent and involves activating the IKK complex, the IKBKB-containing complex that phosphorylates inhibitors of NF-kappa-B.,PTM:Autophosphorylated on serine and threonine residues.,sequence caution:Translated as Tyr.,sequence caution:Wrong choice of CDS.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Homodimer; forms dimers through the leucine-zipper motif. Interacts with the C-terminus of MAPK8IP
- 细胞定位:
- Cytoplasm . Membrane ; Peripheral membrane protein .
- 组织表达:
- Expressed in the adult brain, liver, placenta and pancreas, with expression strongest in the pancreas.
![](/uploadfiles/PIC/yt2610-wb.jpg)
- Western Blot analysis of various cells using LZK Polyclonal Antibody
![](/uploadfiles/PIC/yt2610-wb(2).jpg)
- Western Blot analysis of HepG2 cells using LZK Polyclonal Antibody
![](/uploadfiles/PIC/yt2610_pic486318522.jpg)
- Western blot analysis of lysates from HeLa, HUVEC, and HepG2 cells, using M3K13 Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt2610_pic1008118522.jpg)
- Western blot analysis of the lysates from HepG2 cells using M3K13 antibody.
![](/uploadfiles/PIC/18-ihcbiantaotim20220713.jpg)
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).