LPAAT-δ Polyclonal Antibody
- 货号：YT2582
- 应用：WB;IHC
- 种属：Human;Mouse;Rat
- 简介：
- >>Glycerolipid metabolism;>>Glycerophospholipid metabolism;>>Metabolic pathways;>>Phospholipase D signaling pathway
- 蛋白名称：
- 1-acyl-sn-glycerol-3-phosphate acyltransferase delta
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human AGPAT4. AA range:151-200
- 特异性：
- LPAAT-δ Polyclonal Antibody detects endogenous levels of LPAAT-δ protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000;IHC 1:50-300
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- AGPAT4;1-acyl-sn-glycerol-3-phosphate acyltransferase delta;1-acylglycerol-3-phosphate O-acyltransferase 4;1-AGP acyltransferase 4;1-AGPAT 4;Lysophosphatidic acid acyltransferase delta;LPAAT-delta
- 背景：
- This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. This integral membrane protein converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. [provided by RefSeq, Jul 2008],
- 功能：
- catalytic activity:Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.,domain:The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.,function:Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.,pathway:Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.,similarity:Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.,
- 细胞定位：
- Endoplasmic reticulum membrane ; Multi-pass membrane protein .
- 组织表达：
- Widely expressed with highest levels in skeletal muscle, followed by heart, liver, prostate and thymus.
- Western Blot analysis of various cells using LPAAT-δ Polyclonal Antibody diluted at 1:500
- Western blot analysis of lysates from HeLa and MCF-7 cells, using AGPAT4 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HeLa cells using AGPAT4 antibody.
- Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
