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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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LGR6 Polyclonal Antibody
- 货号:YT2557
- 应用:WB;IHC
- 种属:Human;Rat;Mouse;
- 简介:
- >>Wnt signaling pathway
- 蛋白名称:
- Leucine-rich repeat-containing G-protein coupled receptor 6
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human LGR6. AA range:471-520
- 特异性:
- LGR6 Polyclonal Antibody detects endogenous levels of LGR6 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- LGR6;Leucine-rich repeat-containing G-protein coupled receptor 6
- 背景:
- This gene encodes a member of the leucine-rich repeat-containing subgroup of the G protein-coupled 7-transmembrane protein superfamily. The encoded protein is a glycoprotein hormone receptor with a large N-terminal extracellular domain that contains leucine-rich repeats important for the formation of a horseshoe-shaped interaction motif for ligand binding. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008],
- 功能:
- function:Orphan receptor.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the G-protein coupled receptor 1 family.,similarity:Contains 16 LRR (leucine-rich) repeats.,
- 细胞定位:
- Cell membrane ; Multi-pass membrane protein .
- Western Blot analysis of various cells using LGR6 Polyclonal Antibody diluted at 1:500
- Immunofluorescence analysis of LOVO cells, using LGR6 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HUVEC cells, using LGR6 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).