Laminin α-5 Polyclonal Antibody
- 货号：YT2527
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse
- 简介：
- >>PI3K-Akt signaling pathway;>>Focal adhesion;>>ECM-receptor interaction;>>Toxoplasmosis;>>Amoebiasis;>>Human papillomavirus infection;>>Pathways in cancer;>>Small cell lung cancer
- 蛋白名称：
- Laminin subunit alpha-5
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human LAMA5. AA range:2381-2430
- 特异性：
- Laminin α-5 Polyclonal Antibody detects endogenous levels of Laminin α-5 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- LAMA5;KIAA0533;KIAA1907;Laminin subunit alpha-5;Laminin-10 subunit alpha;Laminin-11 subunit alpha;Laminin-15 subunit alpha
- 背景：
- This gene encodes one of the vertebrate laminin alpha chains. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. The protein encoded by this gene is the alpha-5 subunit of of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523). [provided by RefSeq, Jun 2013],
- 功能：
- domain:Domain G is globular and is part of the major cell-binding site located in the long arm of the laminin heterotrimer.,function:Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.,similarity:Contains 1 laminin IV type A domain.,similarity:Contains 1 laminin N-terminal domain.,similarity:Contains 22 laminin EGF-like domains.,similarity:Contains 5 laminin G-like domains.,subcellular location:Major component.,subunit:Laminin-15 complex is an heterotrimer composed of three chains (alpha-5/beta-2/gamma-3) which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end.,tissue specificity:Expressed in heart, lung, kidney, skeletal mus
- 细胞定位：
- Secreted, extracellular space, extracellular matrix, basement membrane. Major component.
- 组织表达：
- Expressed in heart, lung, kidney, skeletal muscle, pancreas, retina and placenta. Little or no expression in brain and liver.
- Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
- Western Blot analysis of RAW HepG2 A549 cells using Laminin α-5 Polyclonal Antibody
- Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using LAMA5 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°over night
