Ku-70 Polyclonal Antibody
- 货号:YT2498
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 简介:
- >>Non-homologous end-joining
- 蛋白名称:
- X-ray repair cross-complementing protein 6
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human Ku70. AA range:560-609
- 特异性:
- Ku-70 Polyclonal Antibody detects endogenous levels of Ku-70 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- XRCC6;G22P1;X-ray repair cross-complementing protein 6;5'-deoxyribose-5-phosphate lyase Ku70;5'-dRP lyase Ku70;70 kDa subunit of Ku antigen;ATP-dependent DNA helicase 2 subunit 1;ATP-dependent DNA helicase II 70 kDa subunit;CTC box-
- 背景:
- The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008],
- 功能:
- developmental stage:Expression does not increase during promyelocyte differentiation.,disease:Individuals with systemic lupus erythematosus (SLE) and related disorders produce extremely large amounts of autoantibodies to p70 and p86. Existence of a major autoantigenic epitope or epitopes on the C-terminal 190 amino acids of p70 containing the leucine repeat. The majority of autoantibodies to p70 in most sera from patients with SLE seem to be reactive with this region.,function:Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The Ku p70/p86
- 细胞定位:
- Nucleus . Chromosome .
- 组织表达:
- Brain,Cervix carcinoma,Epithelium,Heart,Hepatocyte,Kidney,Liver,Lun
![](/uploadfiles/PIC/yt2498-wb.jpg)
- Western Blot analysis of various cells using Ku-70 Polyclonal Antibody
![](/uploadfiles/PIC/yt2498_pic454718522.jpg)
- Immunofluorescence analysis of COS7 cells, using Ku70 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt2498_pic454818522.jpg)
- Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Ku70 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt2498_pic454918522.jpg)
- Western blot analysis of lysates from HepG2 cells, using Ku70 Antibody. The lane on the right is blocked with the synthesized peptide.