KIF4A Polyclonal Antibody
- 货号：YT2472
- 应用：WB;ELISA;IHC
- 种属：Human
- 蛋白名称：
- Chromosome-associated kinesin KIF4A
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human KIF4A. AA range:1171-1220
- 特异性：
- KIF4A Polyclonal Antibody detects endogenous levels of KIF4A protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- KIF4A;KIF4;Chromosome-associated kinesin KIF4A;Chromokinesin-A
- 背景：
- kinesin family member 4A(KIF4A) Homo sapiens This gene encodes a member of the kinesin 4 subfamily of kinesin related proteins. The encoded protein is an ATP dependent microtubule-based motor protein that is involved in the intracellular transport of membranous organelles. This protein also associates with condensed chromosome arms and may be involved in maintaining chromosome integrity during mitosis. This protein may also be involved in the organization of the central spindle prior to cytokinesis. A pseudogene of this gene is found on chromosome X.[provided by RefSeq, Mar 2010],
- 功能：
- function:Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the kinesin-like protein family.,similarity:Belongs to the kinesin-like protein family. Chromokinesin subfamily.,similarity:Contains 1 kinesin-motor domain.,subcellular location:Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stag
- 细胞定位：
- Nucleus matrix . Cytoplasm . Cytoplasm, cytoskeleton, spindle . Midbody . Chromosome . Associates with chromosomes at all stage of mitosis (PubMed:11736643, PubMed:15297875, PubMed:15625105). Chromatin localization is dependent on iron-sulfur cluster binding (PubMed:29848660). In anaphase, associates with the mitotic spindle midzone (PubMed:15297875). In telophase and cytokinesis, co-localizes with CIAO2B at the spindle midzone and midbody (PubMed:29848660, PubMed:15297875). Co-localizes with PRC1 in early mitosis and at the spindle midzone from anaphase B to telophase (PubMed:15297875, PubMed:15625105). Does not localize to the nucleolus (PubMed:11736643). .
- 组织表达：
- Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung.
- Western Blot analysis of various cells using KIF4A Polyclonal Antibody diluted at 1:500
- Western blot analysis of lysates from HeLa cells, using KIF4A Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HeLa cells using KIF4A antibody.
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
