IRAK-M Polyclonal Antibody
- 货号:YT2393
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse
- 简介:
- >>Neurotrophin signaling pathway
- 蛋白名称:
- Interleukin-1 receptor-associated kinase 3
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human IRAK3. AA range:491-540
- 特异性:
- IRAK-M Polyclonal Antibody detects endogenous levels of IRAK-M protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- IRAK3;Interleukin-1 receptor-associated kinase 3;IRAK-3;IL-1 receptor-associated kinase M;IRAK-M
- 背景:
- This gene encodes a member of the interleukin-1 receptor-associated kinase protein family. Members of this family are essential components of the Toll/IL-R immune signal transduction pathways. This protein is primarily expressed in monocytes and macrophages and functions as a negative regulator of Toll-like receptor signaling. Mutations in this gene are associated with a susceptibility to asthma. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010],
- 功能:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,caution:Ser-293 is present instead of the conserved Asp which is expected to be an active site residue. Low level autophosphorylation activity has been reported in PubMed:10383454, while other authors describe this as an inactive kinase.,cofactor:Magnesium.,disease:Defects in IRAK3 are associated with susceptibility to asthma-related traits type 5 (ASRT5) [MIM:611064]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing, dyspnea, bronchial hyperresponsiveness as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis.,function:Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex.,similarity:Belongs to the protein kinase superfamily. TK
- 细胞定位:
- Cytoplasm . Nucleus . In dendritic cells, translocates into the nucleus upon IL33 stimulation. .
- 组织表达:
- Expressed in eosinophils, dendritic cells and/or monocytes (at protein level) (PubMed:29686383). Expressed predominantly in peripheral blood lymphocytes (PubMed:10383454).
![](/uploadfiles/PIC/yt2393-wb.jpg)
- Western Blot analysis of LOVO cells using IRAK-M Polyclonal Antibody
![](/uploadfiles/PIC/yt2393_pic592718522.jpg)
- Immunofluorescence analysis of HeLa cells, using IRAK3 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt2393_pic592818522.jpg)
- Immunohistochemistry analysis of paraffin-embedded human brain, using IRAK3 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt2393_pic592918522.jpg)
- Western blot analysis of lysates from K562 cells, using IRAK3 Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt2393_pic1003318522.jpg)
- Western blot analysis of the lysates from HUVECcells using IRAK3 antibody.