IRAK-1 Polyclonal Antibody
- 货号:YT2391
- 应用:WB;IHC
- 种属:Human;Mouse;Rat
- 简介:
- >>MAPK signaling pathway;>>NF-kappa B signaling pathway;>>Toll-like receptor signaling pathway;>>Neurotrophin signaling pathway;>>Alcoholic liver disease;>>Pathogenic Escherichia coli infection;>>Salmonella infection;>>Pertussis;>>Yersinia infection;>>Leishmaniasis;>>Chagas disease;>>Toxoplasmosis;>>Tuberculosis;>>Hepatitis B;>>Measles;>>Herpes simplex virus 1 infection;>>Epstein-Barr virus infection;>>Human immunodeficiency virus 1 infection;>>Coronavirus disease - COVID-19;>>Lipid and atherosclerosis
- 蛋白名称:
- Interleukin-1 receptor-associated kinase 1
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human IRAK1. AA range:353-402
- 特异性:
- IRAK-1 Polyclonal Antibody detects endogenous levels of IRAK-1 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- IRAK1;IRAK;Interleukin-1 receptor-associated kinase 1;IRAK-1
- 背景:
- This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
- 功能:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.,PTM:Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.,similarity:
- 细胞定位:
- Cytoplasm . Nucleus . Lipid droplet . Translocates to the nucleus when sumoylated. RSAD2/viperin recruits it to the lipid droplet (By similarity). .
- 组织表达:
- Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
![](/uploadfiles/PIC/yt2391-wb.jpg)
- Western Blot analysis of various cells using IRAK-1 Polyclonal Antibody
![](/uploadfiles/PIC/yt2391_pic276818522.jpg)
- Western blot analysis of lysates from HeLa cells, using IRAK1 Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/175feilinaicc.jpg)
- Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).