HSP40 Polyclonal Antibody
- 货号:YT2254
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 简介:
- >>Protein processing in endoplasmic reticulum;>>Influenza A
- 蛋白名称:
- DnaJ homolog subfamily B member 1
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human HSP40. AA range:271-320
- 特异性:
- HSP40 Polyclonal Antibody detects endogenous levels of HSP40 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- DNAJB1;DNAJ1;HDJ1;HSPF1;DnaJ homolog subfamily B member 1;DnaJ protein homolog 1;Heat shock 40 kDa protein 1;HSP40;Heat shock protein 40;Human DnaJ protein 1;hDj-1
- 背景:
- This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015],
- 功能:
- function:Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.,induction:By heat shock.,similarity:Contains 1 J domain.,subcellular location:Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.,subunit:Interacts with DNAJC3.,
- 细胞定位:
- Cytoplasm . Nucleus . Nucleus, nucleolus . Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.
- 组织表达:
- Brain,Heart,Lung,Placenta,
![](/uploadfiles/PIC/d3142.jpeg)
- Immunofluorescence analysis of Hela cell. 1,HSP40 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Caspase-8 Monoclonal Antibody(2G12)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
![](/uploadfiles/PIC/yt2254-wb.jpg)
- Western Blot analysis of various cells using HSP40 Polyclonal Antibody
![](/uploadfiles/PIC/yt2254_pic449818522.jpg)
- Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using HSP40 Antibody. The picture on the right is blocked with the synthesized peptide.