HSP10 Polyclonal Antibody
- 货号:YT2248
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- 10 kDa heat shock protein mitochondrial
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human HSP10. AA range:51-100
- 特异性:
- HSP10 Polyclonal Antibody detects endogenous levels of HSP10 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- HSPE1;10 kDa heat shock protein; mitochondrial;Hsp10;10 kDa chaperonin;Chaperonin 10;CPN10;Early-pregnancy factor;EPF
- 背景:
- This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. Naturally occurring read-through transcription occurs between this locus and the neighboring locus MOBKL3.[provided by RefSeq, Feb 2011],
- 功能:
- function:Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.,induction:By stress.,mass spectrometry: PubMed:7912672,similarity:Belongs to the groES chaperonin family.,subunit:Homohexamer.,
- 细胞定位:
- Mitochondrion matrix.
- 组织表达:
- B-cell lymphoma,Uterus,
- Western Blot analysis of 3T3 cells using HSP10 Polyclonal Antibody
- Immunofluorescence analysis of NIH/3T3 cells, using HSP10 Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HSP10 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from NIH/3T3 cells, using HSP10 Antibody. The lane on the right is blocked with the synthesized peptide.