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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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GS28 Polyclonal Antibody
- 货号:YT2078
- 应用:WB;ELISA
- 种属:Human;Rat;Mouse;
- 简介:
- >>SNARE interactions in vesicular transport
- 蛋白名称:
- Golgi SNAP receptor complex member 1
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human GOSR1. AA range:11-60
- 特异性:
- GS28 Polyclonal Antibody detects endogenous levels of GS28 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- GOSR1;GS28;Golgi SNAP receptor complex member 1;28 kDa Golgi SNARE protein;28 kDa cis-Golgi SNARE p28;GOS-28
- 背景:
- This gene encodes a trafficking membrane protein which transports proteins among the endoplasmic reticulum and the Golgi and between Golgi compartments. This protein is considered an essential component of the Golgi SNAP receptor (SNARE) complex. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
- 功能:
- function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport.,similarity:Belongs to the GOSR1 family.,subcellular location:Enriched on vesicular components at the terminal rims of the Golgi.,subunit:Interacts with GABARAPL2. Identified in a unique SNARE complex composed of the Golgi SNAREs GOSR2, STX5 and YKT6.,
- 细胞定位:
- Golgi apparatus membrane ; Single-pass type IV membrane protein . Localizes throughout the Golgi apparatus, with lowest levels in the trans-Golgi network (By similarity). Enriched on vesicular components at the terminal rims of the Golgi. Found in Golgi microtubules at low temperature (15 degrees Celsius). .
- 组织表达:
- Colon carcinoma,Prostate,Testis,Umbilical cord blood,
- Western blot analysis of lysates from COLO and K562 cells, using GOSR1 Antibody. The lane on the right is blocked with the synthesized peptide.