GRK 3 Polyclonal Antibody
- 货号：YT2068
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse;Rat
- 简介：
- >>Chemokine signaling pathway;>>Endocytosis;>>Hedgehog signaling pathway;>>Glutamatergic synapse;>>Olfactory transduction;>>Morphine addiction
- 蛋白名称：
- Beta-adrenergic receptor kinase 2
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human GRK3. AA range:361-410
- 特异性：
- GRK 3 Polyclonal Antibody detects endogenous levels of GRK 3 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- ADRBK2;BARK2;GRK3;Beta-adrenergic receptor kinase 2;Beta-ARK-2;G-protein-coupled receptor kinase 3
- 背景：
- The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function. [provided by RefSeq, Jul 2008],
- 功能：
- catalytic activity:ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate.,function:Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 RGS domain.,
- 细胞定位：
- Cell junction, synapse, postsynapse . Cell junction, synapse, presynapse .
- Western Blot analysis of various cells using GRK 3 Polyclonal Antibody diluted at 1:500
- Western blot analysis of lysates from HepG2 and HeLa cells, using GRK3 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from K562 cells using GRK3 antibody.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
